Fig 6.

OM-85 inhibits entry of S protein–pseudotyped lentiviral particles into Vero E6 cells. A, Vero E6 cells were treated with OM-85 (0.48 mg/mL) or PBS for 48 hours, washed, and transduced with lentiviral particles that were pseudotyped with SARS-CoV-2 S protein (0.2 × 10⁴ TU) or VSV-G protein (2 × 10⁴ TU) and carried a GFP reporter. Shown are frames representative of GFP immunofluorescence under various experimental conditions. B-E, GFP fluorescence was assessed under a microscope (10× magnification) 72 hours posttransduction and quantified using the Slide Analyzer software in Vero E6 (Fig 6, B and C) and ACE2/HEK293T (Fig 6, D and E) cells transduced with either SARS-CoV-2-S–pseudotyped virus (Fig 6, B and D) or VSV-G–pseudotyped virus (Fig 6, C and E). Results represent the average ± SEM of 20 (SARS-CoV-2–S-pseudotyped virus) or 10 (VSV-G–pseudotyped virus) random frames from 1 representative experiment of 2 independent experiments. A Wilcoxon 2-sample test was used for statistical analysis after assessing the normality of sample distribution.