Fig E1.

OM-85–induced downregulation of Ace2 and Tmprss2 in the mouse lung is Myd88/Trif-dependent. A, WT and Myd88^−/−Trif^−/− C57BL/6 mice were treated intranasally with OM-85 (1 mg in 50 μL) or PBS every 2 days for 4 times as indicated in the chart (T₁-T₄). Lung cells for transcriptional analyses were collected at day 7. B,Ace2 and Tmprss2 mRNA levels in the lungs of WT and Myd88^−/−Trif^−/− C57BL/6 mice treated intranasally with OM-85 or PBS were measured by RT-qPCR and normalized by Gapdh (n = 2 mice each/PBS group, and n = 3 mice each/OM-85 group). GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; RT-qPCR, RT-quantitative PCR; WT, wild-type.