. 2021 Dec 9;11:23761. doi: 10.1038/s41598-021-03186-y

Table 1.

Localization of SNPs and primer sequences for the tested genes.

Gene	Location	Primer Sequence (5′–3′)	Annealing temperature
SCD1 Chromosome 22	Promoter region (31 C > A)	F: CAGGGGCAGGGGCAGAGGCA R: CGCTGGCAGCCGGTGACTGTG	62 °C
FASN Chromosome 11	Exon 32 (257 C > T)	F: TGAGATGGGGCAGCAGGCCT R: GGAACACTGTTCGCTTGCGGG	60 °C
ANXA9 Chromosome 1	Intron 4 (c.172 + 181G > A) Intron 4 (c.173-27C > G) Intron 5 (c.267 + 103C > A)	F: CATTCCTGTGTGTCCGGTAC R: TCATCTCAGACCTAACCACCA	50 °C

Gene

Location

Primer Sequence (5′–3′)

Annealing temperature

SCD1

Chromosome 22

Promoter region (31 C > A)

F: CAGGGGCAGGGGCAGAGGCA

R: CGCTGGCAGCCGGTGACTGTG

62 °C

FASN

Chromosome 11

Exon 32 (257 C > T)

F: TGAGATGGGGCAGCAGGCCT

R: GGAACACTGTTCGCTTGCGGG

60 °C

ANXA9

Chromosome 1

Intron 4 (c.172 + 181G > A)

Intron 4 (c.173-27C > G)

Intron 5 (c.267 + 103C > A)

F: CATTCCTGTGTGTCCGGTAC

R: TCATCTCAGACCTAACCACCA

50 °C