Fig. 4. CRIg is required for capturing of E. faecalis translocated from gut to the liver.

a and b CRIg^−/− mice and their WT littermates were placed on a chronic–binge ethanol diet and GFP-E. faecalis (2 × 10⁷ CFUs) was intravenously injected 1 h after ethanol-binge. Mice were sacrificed 1 h after injection and Kupffer cells were isolated. a Quantification of the stained cells. b Representative microscopy pictures. c and d CRIg^−/− mice and their WT littermates were fed the chronic-binge ethanol diet. One hour after the ethanol binge, mice were gavaged with E. faecalis (5 × 10⁹ CFUs). Bacterial load (c) and DNA levels (d) were determined 20 min after bacterial gavage. Scale bar = 10 μm. Results are expressed as mean ± s.e.m. (a, c, d). P values determined by two-sided Mann–Whitney test (a, c, d). *P < 0.05, **P < 0.01.