Fig. 5. Extracellular region of CRIg protects mice from ethanol-induced liver disease.

WT mice were fed the chronic–binge ethanol diet. a One hour after the ethanol binge, mice were given intravenous injections of control-Ig or CRIg-Ig (12 mg/kg body weight [BW]) and gavaged with E. faecalis (5 × 10⁹ CFUs). Bacterial load was determined 20 min after bacterial gavage. b–k One day before the ethanol binge, mice were given intravenous injections of control-Ig or CRIg-Ig (12 mg/kg body weight [BW]). b Serum levels of ALT. c Hepatic triglyceride content. d Representative oil red O-stained liver sections. e–g Hepatic levels of mRNAs encoding inflammatory cytokines and chemokines IL1B, CXCL1, and CXCL2. h Serum levels of inflammatory cytokines and chemokines TNF and CXCL1. i Hepatic level of mRNA encoding CRIg. j Serum levels of ethanol. k Hepatic levels of Adh1 and Cyp2e1 mRNAs. Scale bar = 100 μm. Results are expressed as mean ± s.e.m (a–c, e–k). P values determined by two-sided Student’s t-test (a–c, e–k). *P < 0.05, **P < 0.01.