Table 1.

Predicted phenotypes of some gene deletion mutants and comparison with data.

No	Genes or gene family	Proteins or complex	Mutant	p53-wt Cell phenotypea	p53-null Cell phenotypea	p53-wt Plk1-depleted cell phenotypea	p53-null Plk1-depleted cell phenotypea	Data and source
1	CCNB1 & CCNB2	Cyclin B	Cyclin B-null	Inviable	Inviable	Inviable	Inviable	Inviable65,66
2	CDKN1A	p21	p21-null	Viable Tm/T = 1.0	Viable Tm/T = 1.0	Viable Tm/T = 1.3	Viable Tm/T = 1.9	Viable67
3	CDC25	Cdc25	cdc25-null	Inviable	Inviable	Inviable	Inviable	Inviable68
4	WEE1	Wee1	wee1-null	Inviable	Inviable	Inviable	Inviable	Inviable69
5	PLK1	Plk1	plk1-null	Inviable	Inviable	Inviable	Inviable	Inviable55
6	PP2A	PP2A	PP2A-null	Inviable	Inviable	Inviable	Inviable	Inviable70
7	ANAPC1	APC/C	APC-null	Inviable	Inviable	Inviable	Inviable	Inviable71
8	CDC20	Cdc20	cdc20 -null	Inviable	Inviable	Inviable	Inviable	Inviable72
9	CDH1	Cdh1	cdh1-null	Viable Tm/T = 0.3	Viable Tm/T = 0.3	Viable Tm/T = 0.3	Inviable	Suppression by RNAi is viable73
10	PTTG1	Pttg1	pttg1-null	Viable Tm/T = 1.1	Viable Tm/T = 1.1	Viable Tm/T = 1.6	Viable Tm/T = 2.1	Viable74
11	ATM	ATM	ATM-null	Viable Tm/T = 1.0	Viable Tm/T = 1.0	Viable Tm/T = 1.4	Viable Tm/T = 1.4	Viable75
12	TP53	p53	p53-null	Viable Tm/T = 1.0	Viable Tm/T = 1.0	Viable Tm/T = 1.8	Viable Tm/T = 1.8	Viable55
13	MAD2L1	Mad2	mad2-null	Viable Tm/T = 1.0	Viable Tm/T = 1.0	Viable, Tm/T = 1.4	Viable Tm/T = 1.8	Viable76
14	CDH1 & CDKN1A	Cdh1 & p21	cdh1-null p21-null	Inviable	Inviable	Inviable	Inviable	Prediction
15	CDKN1A PP2A	p21 & PP2A	p21-null PP2A-null	Inviable	Inviable	Inviable	Inviable	Prediction
16	CDKN1A WEE1	p21 & Wee1	p21-null wee1-null	Inviable	Inviable	Inviable	Inviable	Prediction
17	CDKN1A PTTG1	p21 & Pttg1	p21-null pttg1-null	Viable Tm/T = 1.1	Viable Tm/T = 1.1	Viable Tm/T = 1.6	Viable Tm/T = 2.2	Prediction
18	CDH1 MAD2	Cdh1 & Mad2	cdh1-null mad2-null	Inviable	Inviable	Viable Tm/T = 0.3	Viable Tm/T = 0.3	Prediction
19	CDC20 PTTG1	Cdc20 & Pttg1	cdc20-null pttg1-null	Inviable	Inviable	Inviable	Inviable	Prediction
20	CDC20 MAD2	Cdc20 & Mad2	cdc20-null mad2-null	Inviable	Inviable	Inviable	Inviable	Prediction
21	PP2A MAD2	PP2A & Mad2	PP2A-null mad2-null	Inviable	Inviable	Inviable	Inviable	Prediction
22	PP2A PTTG1	PP2A & Pttg1	PP2A-null pttg1-null	Inviable	Inviable	Inviable	Inviable	Prediction
23	CDC25 WEE1	Cdc25 & Wee1	cdc25-null wee1-null	Inviable	Inviable	Inviable	Inviable	Prediction

^aT_m/T is the ratio of cell cycle periods observed in the mutants T_m and in wild-type cancer cells T.