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. 2021 Dec 9;12:7151. doi: 10.1038/s41467-021-27461-8

Fig. 1. Structural remodeling of the adult plna R14del zebrafish heart.

Fig. 1

AC Bright-field images of isolated adult zebrafish hearts, 2 years of age: wild-type fish, remodeled plna R14del mutant heart and non-remodeled plna R14del heart. DF Hematoxylin and eosin staining of the three conditions to identify nuclei, with zoom-in at indicated regions. GI Picro-sirius red staining of collagen deposition for the three conditions, collagen fibers are shown as red staining. JL Oil Red O staining for fat/lipid of the three conditions. All stainings were performed on WT n = 3, plna R14del n = 3, two experimental replicates. Zoom-in of each indicated region is included. Images were taken at a magnification of ×20. Scale bars are 200 µm for whole-heart tile scans and 50 µm for zoom-in regions. A atrium, V ventricle, BA bulbus arteriosus.