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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Curr Opin Chem Biol. 2021 Jun 10;65:42–48. doi: 10.1016/j.cbpa.2021.04.012

Figure 2.

Figure 2.

A. Chemical structure of tri-functional phosphatidylinositol (PI). Accumulation of PI in endo membranes after uncaging for 5 sec (B) or 2 min (C) followed by 2 min of photo-crosslinking. The location of the lipid was monitored by confocal microscopy after fixation and labelling the terminal alkyne of the lipid tail with an azide-bearing fluorophore via copper click chemistry.