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. 2021 Nov 26;9:781365. doi: 10.3389/fcell.2021.781365

FIGURE 2.

FIGURE 2

Reversible GSDME upregulation during EMT. (A) Three indicated lung cancer cell lines were treated with TGFβ (5 ng/μl) at a time course manner and analyzed by Western blot for GSDME protein expression. (B) Imaging analysis of lung cancer cells showed EMT morphology upon TGFβ treatment (scale bar = 50 μm). (C) Three indicated ovarian cancer cell lines were treated with TGFβ (5 ng/μl) at a time course manner and analyzed by Western blot for GSDME protein expression. (D) Imaging analysis of ovarian cancer cells showed EMT morphology upon TGFβ treatment (scale bar = 50 μm). (E) Osimertinib-resistant NCI-H1975 (OR) and erlotinib-resistant HCC827 (ER) cells were generated by long-term culture and analyzed by Western blot for GSDME protein expression. (F) NCI-H1975 and HCC827 cells were treated with TGFβ (5 ng/μl) to induce EMT. TGFβ was withdrawn as indicated and cells were analyzed by Western blot for GSDME protein expression.