FIGURE 6.

Working model for synuclein’s role in SV clustering. (A) At resting, intact synapses, synuclein is localized to the SV clusters. The distal reserve pool of SVs is denoted by the light green region, and the pool of docked SVs is denoted by the dark green region. (B) After synuclein disruption using the pan-synuclein antibody, SVs in the distal reserve pool disperse into smaller subclusters, as well as individual SVs. The remaining SV subclusters may be held together via synapsin-mediated LLPS or some other scaffolding mechanism involving protein tethers. After synuclein disruption, SVs nearest the active zone are also depleted, indicating a role for synuclein in SV docking.