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. 2021 Nov 25;8:763902. doi: 10.3389/fmolb.2021.763902

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Copyright © 2021 Han, Li, Chen and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Steps for studying cancer metabolism using stable isotope-resolved metabolomics (SIRM). Stable isotope tracers, such as uniformly ¹³C-labeled glucose (¹³C₆-Glc) or uniformly ¹³C,¹⁵N-labeled glutamine (¹³C₅,¹⁵N₂-Gln), are administered via addition to the culture medium for cells or via intravenous injection into whole organisms. Metabolites are then extracted, labeled by tracers, and subjected to various NMR and MS analyses to probe metabolic activity.