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A–D: TaqMan quantitative RT-PCR of the isoforms in islets (ISL) and ChP. A: INS pORF isoforms in control (CNT) (n = 13) and T2DM islets (n = 9) using INS1B as a reference. B: INS pORF isoforms in human islets (n = 13) and ChP (n = 15) using exon 2 as a reference. C: Comparison of INS isoforms in ChP samples from CNT, T1DM, and T2DM. D: ddPCR quantification of INS uORF isoforms in ISL and ChP. B2M, β2 microglobulin.
