Figure 3.

Blood CD8⁺ T cells recognize either R- or X-GRP78 peptide versions in adolescents with type 1 diabetes. A: Frequencies of MMr⁺CD8⁺ cells reactive exclusively to the GRP78-R (blue symbols) or GRP78-X (red symbols) peptides, or to both (green symbols), in HLA-A2⁺ adolescents with type 1 diabetes (T1D) (n = 8). PPI_6–14 and Flu MP_58–66 peptides were included as controls. At least 0.39 × 10⁶ CD8⁺ T cells were counted for each donor (median 1.1 × 10⁶ [range 0.39–2.4 × 10⁶]). B: Percentage of antigen-experienced cells out of total MMr⁺ cells for the GRP78 peptides depicted in A. Data points with <5 MMr⁺ cells were excluded (median 15 MMr⁺ cells [range 5–115] for GRP78 peptides). Bars show median values. NA, not available (i.e., <5 MMr⁺ cells counted). *P < 0.05, **P < 0.01, ***P < 0.001 by Mann-Whitney U test.