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. 2021 Nov 8;297(6):101391. doi: 10.1016/j.jbc.2021.101391

Figure 1.

Figure 1

Structural characterization of oncofetal CS.A, diagram representing intact oncofetal CS (I-ofCS), ChABC retained ofCS (R-ofCS), and ChABC liberated ofCS (L-ofCS) purified using rVAR2 affinity chromatography. B, disaccharide (dp2) structure analysis of placental CS and placental ofCS, analyzed by LC-MS (N = 3). C, dp2 analysis of colon tumor CS and R-ofCS (N = 1). D, MS analysis and composition of full-length R-ofCS (supplemental methods). E, AMAC-labeled R-ofCS was loaded on a rVAR2-column and eluted with a gradient of sodium chloride. R-ofCS elution was detected at 0.46 M NaCl. R-ofCS was fractionated with different concentrations of NaCl and subsequently (F) quantified and (G) structurally characterized by disaccharide analysis using LC-MS (N = 3). Error bars indicate mean ± SEM.