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. 2021 Nov 8;297(6):101391. doi: 10.1016/j.jbc.2021.101391

Figure 4.

Figure 4

Impact of CHPF and CHPF2 inactivation on rVAR2 binding and CS chain length.A, rVAR2 binding to HeLa WT and mutant cell lines, analyzed by flow cytometry (N = 3). B, structural analysis and (C) quantification of cell surface CS from HeLa WT and mutant cell lines, analyzed by LC-MS (N = 3). D, size exclusion chromatogram of radiolabeled cell surface CS from WT and mutant cell lines, separated using a 1.5 × 50 cm column packed with Sepharose 6B (N = 2). Error bars indicate mean ± SEM. ∗∗∗p < 0.001 and ∗p < 0.05 by unpaired t test.