Table 1.
Details of ELISA kits used to test bulk tank milk samples from study herds between 2018 and 2020.
| Pathogen | Year used | ELISA test kit name | ELISA test kit manufacturer | Reported sensitivity (%) | Reported specificity (%) | Pp cut-off value used |
|---|---|---|---|---|---|---|
| BVDV | 2018 | BVDV p80 Ab | IDEXX Laboratories, Westbrook, MA | 93.6 | 100 | 0.2 |
| 2019, 2020 | ID Screen® BVD p80 Antibody Competition | IDVet, Montpellier, France | 100 | 100 | >65 | |
| L. Hardjo | All sampling periods | Linnodee Leptospira Hardjo ELISA | Linnodee Ltd., Ballyclare, Northern Ireland | 94.1 | 94.8 | >0.1 |
| S. Dublin | All sampling periods | ID Screen® Salmonlla Dublin milk | IDVet, Montpellier, France | 63.2* | 99.1 | ≥70 |
| N. caninum | All sampling periods | ID Screen® Neospora caninum milk | (IDVet, Montpellier, France) | 88 | 89.4 | ≥30 |
| BoHv-1 | All sampling periods | IDEXX IBR Pool Ab Test | IDEXX GmbH, Germany | 90.7 | 99.2 | >25 |
| MAP | All sampling periods | IDEXX MAP Ab Test | IDEXX GmbH, Germany | 56 | 96 | >30 |
| M. bovis | 2018 | BIO K 302 indirect Ab test | BioX Diagnostics, Belgium | 60.4 | 97.3 | ≥37 |
| 2019, 2020 | ID Screen® Mycoplasma bovis antibody ELISA | IDVet, Montpellier, France | 93.5 | 98.6 | ≥30 | |
| BRSV | All sampling periods | SVANOVIR® BRSV-Ab | SVANOVIR® BRSV-Ab ELISA, Boehringer Ingelheim Svanova, Sweden | 94.6 | 100 | ≥10 |
*
Sensitivity information was not available for this ELISA kit. For true prevalence calculations, an Se of 63.2% from a comparable kit was used [Salmonella B/D LPS antibody ELISA (bulk milk), GD Animal Health Services, Netherlands].