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. 2021 Oct 30;297(6):101365. doi: 10.1016/j.jbc.2021.101365

Figure 2.

Figure 2

Western blot analysis of p53, p21, and p14ARF in NARF2 cells.A, NARF2 cells were pretreated with or without IPTG for 24 h and exposed to CETZOLE 1 for another 24 h at indicated concentrations. Western blotting was used to analyze p53, p21, p14ARF, and actin as a loading control. B, time course of p53 expression in NARF2 cells after CETZOLE 1 treatment. Cells were incubated without IPTG and then exposed to CETZOLE 1 (20 μM) for the times indicated. C, p53 expression in cells exposed to different doses of CETZOLE 1 at two time points (24 h and 48 h). NARF2 cells without IPTG were exposed to CETZOLE 1 as indicated and analyzed by Western blotting.