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Expression of p53 and p21 in MDAH01 and HT1080 cells. Western blot analysis of p53 and p21 expression. A, Western blot of MDAH041 cells exposed to Nutlin-3a (Nut) for 24 h. HT1080 were included as a positive control for p53 and p21 expression. Actin was assessed to determine loading. B, Western blot analysis of HT1080-LXSN and HT1080-GSE56 cells exposed to either etoposide or Nutlin-3a (Nut3) for 24 h.
