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. 2021 Oct 30;297(6):101365. doi: 10.1016/j.jbc.2021.101365

Figure 6.

Figure 6

Western blot analysis of CDK, cyclin, and RB. Western blotting was used to measure levels of CDK2, cyclin E, p-RB (ser780), total RB, ACSL4, and actin. NARF2 pBpuro or K2/D cells were infected with adenoviruses that express CDK2, cyclin E, or an empty vector (CMV) at indicated MOIs and incubated for 48 h before Western blot analysis. A, CDK2 and cyclin E were expressed together. B and C, these proteins were expressed separately in NARF2 pBpuro cells for 48 h before Western blot analysis. CDK, cyclin-dependent kinase; RB, retinoblastoma; MOI, multiplicity of infection.