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. 2021 Oct 30;297(6):101365. doi: 10.1016/j.jbc.2021.101365

Figure 9.

Figure 9

RB inhibits ferroptosis. The cells indicated were exposed to either CETZOLE 1 (C1) or RSL3 to induce ferroptosis. Viability was determined 48 h later using methylene blue staining. WT and RB family triple KO (TKO) cells are shown in (A) and (B), whereas cells lacking p107 and p130 (DKO) are shown in (C) and (D). ∗p < 0.05 when comparing KO with the corresponding WT cell line. E–G, MDA MB 468 cells which normally lack p105RB were reconstituted with WT p105RB by stable transfection. A negative control cell line was prepared by stable transfection with empty LXSN plasmid. Clones #1 and #14 expressing RB were exposed to (E) CETZOLE 1 or (F) RSL3 for 72 h and analyzed for viability. ∗p < 0.05 when comparing RB expressing clones with control LXSN cells. G, Western blotting was used to identify clones expressing p105RB. Actin was used as a loading control. p values were generated using the Student's t test. DKO, double KO; RB, retinoblastoma; RSL3, ras-selective lethal 3.