Figure 2.

CTEN overexpression suppresses VEGFA expression in HUVECs. (A) Western blot results showing overexpression of CTEN in HUVEC cells. n = 5. (B) Quantitative real-time PCR showing CTEN mRNA expression. n = 5. (C) Relative VEGFA protein levels were quantified by Western blot. n = 5. (D) Quantitative real-time PCR showing VEGFA mRNA expression. n = 5. (E) Western blot results of CTEN and VEGFA in cells knockdown of CTEN by 2 oligos. n = 5. (F) mRNA expression level of CTEN quantified by real-time RT-PCR. n = 5. (G and H) Expression level of VEGFA quantified by Western blot and real-time RT-PCR. n = 5. **P < .01.

Abbreviations: CTEN, C-terminal tensin-like; HUVEC, human umbilical vein endothelial cell; OE, overexpression; RT-PCR, reverse transcriptase-polymerase chain reaction; VEGFA, vascular endothelial growth factor A.