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Graphical representation of methodology. (A) Extraction of EVs from plasma and characterization and quantification of EVs from different techniques. (B) Isobaric tag for relative and absolute quantitation (ITRAQ) labeling and Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) analysis of EVs proteins. (C) Functional analysis of EVs altered proteins in different grades of Glioma. (D) Verification by Enzyme-linked immunosorbent assay (ELISA).
