TABLE 2.
Primers used to screen for interspecific variation in Malassezia species
| Locus or region | Primer set | Primer sequence (5′-3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| Large-subunit rRNA gene | 26S-Sa | GCTGAACTTAAG | 16 | |
| CATATCAT | ||||
| 26S-Ab | TAGACGTTAGAC | 642 | 11 | |
| TCCTTGGT | ||||
| ITS | ITS 1 | TCCGTAGGTGAA | ∼800/700 | 35 |
| CCTGCGG | ||||
| ITS 4 | TCCTCCGCTTATT | |||
| GATATGC | ||||
| β-Tubulin gene | Bt-2a | GGTAACCAAATC | ∼550 | 12 |
| GGTGCTGCTTTC | ||||
| Bt-2b | ACCCTCAGTGTA | |||
| GTGACCCTTGGC | ||||
| Lipase genec | LIP-F | GTGTTGGCGTAC | ∼530 | 24 |
| CCGTCGTT | ||||
| LIP-R | CGAGGTCGTTGG | |||
| CAAACGCA |
a
The primer designed correspond to nucleotides 1 to 20 of the partial sequence of M. furfur (CBS 1878; accession no. AF063214).
b
The antisense primers for the LSU rRNA gene were designed from the sequence of Saccharomyces cerevisiae (accession no. J01355).
c
Primers for the lipase region were designed from the sequence of the LIP2 gene of C. cylindracea (accession no. X64704). A 700-bp fragment that was conserved among the five accessioned sequences of lipase genes from C. cylindracea was used for designing primers.