TABLE 3.

Molecular differentiation of Malassezia species by PCR-REA

Genomic region	Restriction endonuclease	PCR-REA types (Malassezia speciesa)
26S rRNA gene (LSU)	AvaI	A (Mf, Msy, Msl); A′ (Mf, Msl); B (Mg, Mr, Mo, Mp)
TS (ITS 1-ITS 4)	EcoRI	C (Mf, Mp, Mo); C′ (Msl); D (Mg, Mr); D′b, E (Msy)
	NcoI	F (Mf, Mp); G (Mg, Mr, Mo, Msl); G′c, H (Msy)
β-Tubulin gene		P (Mr, Mf, Msy, Msl, Mp, Mo)d; N (Mg, Mf, Msy, Msl, Mp)e

^aMf, M. furfur; Msl, M. slooffiae; Msy, M. sympodialis; Mp, M. pachydermatis; Mo, M. obtusa; Mg, M. globosa; Mr, M. restricta. 

^bOnly 3 of 37 M. sympodialis strains had this PCR type (see Fig. 2B). 

^cOnly 2 of 37 M. sympodialis strains had this PCR type. Restriction with NcoI produced two bands of 500 and 200 bp (PCR-REA type G′) instead of one 350-bp band of double intensity (PCR-REA type H). 

^dPositive amplification with primers for β-tubulin gene. 

^eNegative amplification with primers for β-tubulin gene.