Table 1.

Photophysical and Binding Properties of Fluorophore-Broccoli Complexes^a

	Fluorophore	Excitation (nm)	Emission (nm)	Extinction Coefficient (M−1, cm−1)b	Quantum Yield	Brightnessc	Kd (nM)
	DFHBI-1T alone	428	500	35,400	0.00041	0.121	N/A
	Broccoli-DFHBI-1T	470	505	28,900	0.41	100	305±39
	DFNS alone	435	527	37,700	0.00031	0.0974	N/A
	Broccoli-DFNS	470	527	31,600	0.22	57.0	646±103
	BI alone	425	500	42,500	0.00048	0.171	N/A
	Broccoli-BI	470	505	33,600	0.67	188	51±5
	TBI alone	433	524	43,300	0.00037	0.133	N/A
	Broccoli-TBI	485	527	35,100	0.64	189	71±18

^a.Photophysical and binding properties of DFHBI-1T alone and Broccoli-DFHBI-1T, BI alone and Broccoli-BI are reported from a previous study.^[3] All data were measured at pH 7.4 at 25°C.

^b.Brightness (extinction coefficient × quantum yield) is relative to Broccoli-DFHBI-1T.^[3]