1,25(OH)2D deficiency inhibits antioxidant capacity of skeletal muscle tissue. (A) Representative micrographs of tibialis anterior muscle sections from 6-month-old WT and Cyp27b1 KO male mice stained with IF for SOD1 and (B) SOD1 positive myofibers (%). (C, D) Western blots for SOD1protein expression in skeletal muscle tissue. (E) mRNA expression levels in skeletal muscle tissue of the antioxidant enzymes CAT, SOD1, Nqo1 and Gcs. Values are means ± SEM of 6 determinations per group. *P<0.05; **P<0.01; ***P<0.001, compared with WT mice.