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. 2021 Nov 15;13(11):12638–12649.

Figure 4.

Figure 4

1,25(OH)2D deficiency induces senescence and SASP in skeletal muscle tissue. (A) Representative micrographs of tibialis anterior muscle sections from 6-month-old WT and Cyp27b1 KO male mice stained with IF for p16 and p21 (B) p16 and p21 positive myofibers (%). (C, D) Western blots for p16, P19, p21 and p53 protein expression in skeletal muscle tissue. (E) mRNA expression levels of p16, p21, TNFα, IL-6 and MMP3 in skeletal muscle tissue. Values are means ± SEM of 6 determinations per group. *P<0.05; **P<0.01, compared with WT mice.