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Id2 causes rapid disruption of Pax-5–DNA complexes. (A) Schematic representation of the experimental protocol used. (B) Gel retardation analysis of binary (2°) complex formation by Pax-5(1-175) and the mb-1 promoter in the presence (lanes 1–5) or absence (lanes 6–10) of added Id2. Equal amounts of rabbit reticulocyte lysate were added to each tube. The times at which samples were withdrawn following Id2 addition to the binding reaction mixture are indicated above each lane.
