Figure 1.

Impact of duration of CA on B. distachyon freezing tolerance. (A) Electrolyte leakage assays conducted using leaf tissue from nonacclimated and cold-acclimated wildtype ecotype Bd21. Days of CA and temperatures are shown. Samples were subjected to the low temperatures indicated at the rate of 1°C every 15 min before assaying for electrolyte leakage (%). (B) Accumulation of soluble sugars in leaf tissue from nonacclimated and cold-acclimated wildtype ecotype Bd21. Days of CA and soluble sugar type are shown in four clusters of histograms and expressed as concentration in leaf fresh weights. Four biological replicates were conducted for all assays (n = 10) and ANOVA and post hoc Tukey’s tests were performed. Error bars represent the standard error of the mean. Letters above the histograms indicate statistically significant groups (P < 0.05) with separate analyses for each temperature and soluble sugar.