Fig. 3.

Affinity assessment of multivalent binding and membrane fluidity effect. Diagrams of RBC-Ab (A), bead-Ab (B), and antibody (C) binding to SW480 cells. Characterization of the dissociation constants of RBC-Ab (D), Bead-Ab (E), and antibody (F) to SW480 cells. (G) Fluorescence images of RBC before blench (a), immediately after bleach with strong light irradiation (488 nm, 8.6 s) (b) and fluorescence restoration after 30s (c). The white box highlighted the change site. (H) Real-time fluorescence intensity of RBC membrane in the area of white box. Vertical dashed line indicates the time to stop the light irradiation.