Figure 1.

Intervenolin (ITV) exhibits anti-cancer activity that is potentiated in co-culture conditions

(A) Structure of ITV.

(B) Growth of MKN-74 cells cultured alone (Mono) or with Hs738 (Co) for 3 days at the indicated ITV concentrations were measured using GFP fluorescence intensity and expressed as percentage relative to that of cells without ITV treatment (n = 3; ∗p < 0.005, ∗∗p < 0.001 versus mono).

(C) Growth of MKN-74 cells upon treatment with ITV CM, Ctrl CM, and their respective controls (DMEM + ITV and Ctrl CM + ITV) for 3 days (∗p < 0.001 versus Ctrl CM+ ITV). For each condition, 1 μg/mL ITV was used.

(D) Growth of cancer cell lines MKN-1, -B, and −74 (n = 3).

(E) Formalin-fixed, paraffin-embedded tumor sections from mice inoculated with MKN-74 and Hs738 cells treated with the vehicle or 12.5 mg/kg ITV were stained with Masson's trichrome stain and analyzed using immunohistochemistry with antibodies against the indicated proteins. Scale bar, 100 μm (n = 5).

(F) Percentages of Ki-67-positive cells in the high-power field of tumor sections (n = 5; ∗p < 0.01).

(G) MKN-74 cells were cultured alone (Mono) or with fibroblast cells isolated from mice tumor tissues (+nude FB74-1,2) (Co) for 3 days in the presence of ITV at the indicated concentrations (n = 3; ∗p < 0.0005 versus mono).

Data are presented as the mean ± SD and were analyzed using two-sided Student's t test.