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. 2021 Nov 26;24(12):103517. doi: 10.1016/j.isci.2021.103517

Figure 5.

Figure 5

HIF-1α positively regulates GFAT2 in cardiomyocyte hypertrophy

(A) Representative immunoblots of HIF-1α, GFAT2, phospho-Akt(S473), Akt, and GAPDH in NRVMs treated with indicated siRNA in the presence or absence of ISO (1 μM, 12 h) (n = 5).

(B–D) Quantitative analysis of protein HIF-1α, GFAT2, and phospho-Akt in NRVMs treated with indicated siRNA in the presence or absence of ISO (1 μM, 12 h) (n = 5).

(E) Expression of GFAT2 and 18S mRNA in NRVMs treated with indicated siRNA in the presence or absence of ISO (1 μM, 12 h) (n = 5).

(F) Cell surface area of NRVMs treated with indicated siRNA in the presence or absence of ISO (1 μM, 12 h) (n = 150 cells in each group). Scale bar, 50 μm. ∗p < 0.05, ∗∗p < 0.01: post hoc Tukey's comparison test.