DON suppresses Akt activation in ISO-induced cardiac hypertrophy
(A) Representative immunoblots of Akt, phospho-Akt(S473), ERK, phosphor-ERK, p38, phosphor-p38, JNK, phospho-JNK, and GAPDH in ISO (15 mg/kg body weight/day)- or control vehicle (saline)- treated C57B/6J mouse hearts subjected to either DON (0.05 mg/kg body weight/day) or saline for 7 days.
(B–E) Quantitative analysis of phospho-Akt, phospho-ERK, phospho-p38, phospho-JNK, and GAPDH in ISO- or control vehicle-treated C57B/6J mouse hearts subjected to either DON (0.05 mg/kg body weight/day) or saline for 7 days (n = 9). ∗p < 0.05: post hoc Tukey's comparison test.