REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-GFAT1	Abcam	Cat#ab125069; RRID: AB_10975709
Rabbit monoclonal anti-GFAT2	Abcam	Cat#ab190966; RRID: AB_2868470
Rabbit monoclonal anti-OGT	Cell Signaling Technology	Cat#24083; RRID: AB_2716710
Rabbit polyclonal anti-OGA	Novus Biologicals	Cat#NBP1-81244; RRID:AB_11053819
Mouse monoclonal anti-O-GlcNAc(CTD110.6)	Cell Signaling Technology	Cat#9875; RRID: AB_10950973
Mouse monoclonal anti-O-GlcNAc(RL2)	Novus Biologicals	Cat#NBP300-524; RRID: AB_10001871
Rabbit monoclonal anti-Akt	Cell Signaling Technology	Cat#4691; RRID: AB_915783
Rabbit monoclonal anti-phospho-Akt(S473)	Cell Signaling Technology	Cat#4060; RRID: AB_2315049
Rabbit monoclonal anti-phospho-Akt(T308)	Cell Signaling Technology	Cat#13038; RRID: AB_2629447
Rabbit monoclonal anti-mTOR	Cell Signaling Technology	Cat#2972; RRID: AB_330978
Rabbit monoclonal anti-phospho-mTOR	Cell Signaling Technology	Cat#5536; RRID: AB_10691552
Rabbit monoclonal anti-S6	Cell Signaling Technology	Cat#2217; RRID: AB_331355
Rabbit monoclonal anti-phospho-S6(Ser235/236)	Cell Signaling Technology	Cat#4858; RRID: AB_916156
Rabbit monoclonal anti-p70 S6 Kinase	Cell Signaling Technology	Cat#2708; RRID: AB_390722
Rabbit monoclonal anti-phospho-p70 S6 Kinase	Cell Signaling Technology	Cat#9234; RRID: AB_2269803
Rabbit polyclonal anti-ERK	Cell Signaling Technology	Cat#9102; RRID: AB_330744
Rabbit polyclonal anti-phospho-ERK	Cell Signaling Technology	Cat#9101
Rabbit polyclonal anti-p38	Cell Signaling Technology	Cat#9212; RRID: AB_330713
Rabbit monoclonal anti-phospho-p38	Cell Signaling Technology	Cat#4511; RRID: AB_2139682
Rabbit polyclonal anti-JNK	Cell Signaling Technology	Cat#9252; RRID: AB_2250373
Rabbit monoclonal anti-phospho-JNK	Cell Signaling Technology	Cat#4668; RRID: AB_823588
Rabbit monoclonal anti-HIF-1α	Cell Signaling Technology	Cat#36169; RRID: AB_2799095
Rabbit monoclonal anti-CaMKII	Cell Signaling Technology	Cat#4436; RRID: AB_10545451
Mouse monoclonal anti-β-Actin	Santa Cruz Biotechnology	Cat#sc47778; RRID:AB_2714189
Mouse monoclonal anti-GAPDH	Santa Cruz Biotechnology	Cat#sc32233; RRID:AB_627679
Rabbit monoclonal anti-Troponin I	Cell Signaling Technology	Cat#13083; RRID: AB_2798114
Fluorescein goat anti-rabbit IgG antibody	Vector Laboratories	Cat#FI-1000; RRID:AB_2336197
Bacterial and virus strains
pAV[Exp]-EGFP-CMV>6xHis/rAkt1(T479A)	VectorBuilder	N/A
pAV[Exp]-EGFP-CMV>6xHis/rAkt1	VectorBuilder	N/A
Chemicals, peptides, and recombinant proteins
Angiotensin II	Sigma Aldrich	Cat#A9525; CAS:4474-91-3
6-Diazo-5-oxo-L-norleucine crystalline (DON)	Sigma Aldrich	Cat#D2141; CAS:157-03-9
D-glucosamine hydrochloride	Sigma Aldrich	Cat#G1514; CAS:66-84-2
MK-2206	Selleck Chemicals	Cat#S1078; CAS:1032350-13-2
Isoproterenol hydrochloride	Tokyo Chemical Industry	Cat#I0260; CAS:51-30-9
Phenylephrine hydrochloride	Sigma Aldrich	Cat#P6126; CAS:61-76-7
Experimental models: Cell lines
C2C12	Masataka Ikeada	NA
HEK293	Masataka Ikeada	NA
Experimental models: Organisms/strains
Mouse C57BL/6, male	CREA Japan	https://www.clea-japan.com/en/products/inbred/item_a0420
Oligonucleotides
qPCR primers	See Table S1	NA
siRNA targeting sequence: GFAT2	Thermo Fisher Scientific	Cat#s165752
siRNA targeting sequence: GFAT1	Thermo Fisher Scientific	Cat#s150682
siRNA targeting sequence: OGT	Thermo Fisher Scientific	Cat#s130676
siRNA targeting sequence: OGA	Thermo Fisher Scientific	Cat#s139145
siRNA targeting sequence: HIF-1α	Takara Bio	Cat#RS0035615
siRNA targeting sequence: CaMKIIγ	Thermo Fisher Scientific	Cat#s139829
siRNA targeting sequence: CaMKIIδ	Thermo Fisher Scientific	Cat#s127546
Software and algorithms
Fusion Capt Advance	Vilber Lourmat	NA
BZ-X800 Analyzer (1.1.1.8)	Keyence corporation	NA
JMP pro 15	SAS Institute	NA
Other
BZ-X800:fluorescent microscope	Keyence corporation	NA
NIBP Monitor for Rats & Mice	Muromachi Kikai	Cat#Model MK-1030
Vevo 2100 ultrasonography system	Visual Sonics	NA