Representative Z-stack rendered biofilm 3D-images of P. aeruginosa on CTL and HTE surfaces for day 7 (Fig. 7A and D, respectively), 14 (Fig. 7B and E, respectively) and 21 (Fig. 7C and F, respectively). Biovolume of P. aeruginosa incubated on CTL and HTE for day 7, 14 and 21 (Fig. 7G) mean ± SD, (n = 3). Yellow arrows highlight pockets of bacterial recolonisation on top of dead biofilm (scale bar = 30 μm), images signify an area of 0.01 mm2 (n = 3). Viability of P. aeruginosa (Fig. 7H) at three time points calculated from biovolume using multiple t-tests followed by the Holm-Sidak method, with alpha = 0.05. Each column represents mean ± SD, (n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)