Fig. 1.

Heme is a more potent platelet agonist than hemoglobin. Platelet activation measured by (A) platelet surface P-selectin or (B) activated GPIIb/IIIa levels in heme (blue line), hemoglobin (red line) treated platelets. (C) Thrombospondin-1 levels quantified in the releasate from heme (blue line) or hemoglobin (red line) treated platelets. (D) Representative dot blot with quantification of IL-1β, CXCL7, FGF basic, PDGF-B, angiostatin, TGFβ, kininogen, CD40L, PAI-1 levels in the releasate from heme (2.5 μM) treated platelets. Data are represented as Mean ± SEM. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns- not significant. n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)