Fig. 2.

Effects of RND3 overexpression (OE) on superoxide production and NOX expressions in VSMCs of WKY and SHR. The VSMCs of WKY and SHR were treated with PBS, negative control (NC) or RND3 overexpressed plasmid (50 nM) for 24 h. A, superoxide level. B, relative DHE fluorescence intensity. C, representative images of DHE fluorescence staining. D, H₂O₂ level. E, NOX activity. F, NOX1, NOX2 and NOX4 mRNA levels. G, NOX1, NOX2 and NOX4 protein expressions. Values are mean ± SE. *P < 0.05 vs WKY; †P < 0.05 vs PBS or NC. n = 6 per group in A-E; n = 3 per group in F. n = 4 per group in G.