Fig. 3.

Roles of RND3 overexpression (OE) in VSMCs migration and proliferation of WKY and SHR. The VSMCs of WKY and SHR were treated with PBS, negative control (NC) or RND3 overexpressed plasmid (50 nM) for 24 h. A, VSMCs migration evaluated with wound healing assay. B, VSMCs migration evaluated with Boyden chamber assay. C, PCNA protein expression. D, percentage of EdU-positive cells. E, representative images of EdU-positive cells. The red fluorescence represents proliferating cells, and the blue fluorescence represents total cells. Values are mean ± SE. *P < 0.05 vs WKY; †P < 0.05 vs PBS or NC. n = 4 per group in C; n = 6 per group in others. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)