Fig. 9.

Roles of adenoviral (Ad)-mediated RND3 overexpression in WKY and SHR in PCNA and ROCK1 protein expressions, phosphorylation of MYPT1 and oxidative stress. The rat was subjected to intravenous administration of PBS, Ad-scrambled RND3 (NC) or Ad-RND3 (RND3 OE, 1 × 10⁷ plaque forming units). The measurements were performed 4 weeks after the intravenous injection. A, ROCK1 protein expression. B, MYPT1 phosphorylation. C, bar graph showing the relative values of the DHE fluorescence staining analyses. D, representative images of DHE fluorescence staining, a marker of ROS production in aorta and MA. E, NOX1, NOX2 and NOX4 mRNA levels. Values are mean ± SE. *P < 0.05 vs WKY; †P < 0.05 vs. PBS or NC. n = 6 per group.