TABLE 2.
Recovery of mitochondrial nucleic acid markers
| Sample | Relative amt of RNA speciesa
|
Mean recovery | |||
|---|---|---|---|---|---|
| mtDNA | 12S rRNA | ND1 mRNA | COII mRNA | ||
| Whole cells | 100 | 100 | 100 | 100 | 100 |
| Mitochondrial fraction | |||||
| Washed | 21.5 | 7.5 | 23.6 ± 2.1 | 19.0 ± 1.2 | 17.9 |
| MN treated | 16 | 4 | 9.2 ± 1.8 | 10.2 ± 0.9 | 9.9 |
| D treated | 18 | 6 | 16.5 ± 2.6 | 14.5 ± 1.0 | 13.7 |
| D plus MN treated | 9 | 3 | 7.7 ± 1.5 | 6.7 ± 1.1 | 6.6 |
a
The data for total cells and the variously treated mitochondrial fractions were normalized to the total nucleic acids recovered in total cells and in each fraction per milliliter of packed cells. The values reported represent the results of single gel determinations for mitochondrial DNA (mtDNA) and 12S rRNA, and the averages (±2 standard errors of the mean) of five independent determinations (from two preparations) for ND1 mRNA and COII mRNA.