TABLE 5.
Virological characteristics of positive cats to reverse transcription quantitative PCR (RT‐qPCR)
| Ct values of qRT‐PCR | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal ID | Species | Type of sampling and origin | Type of sample | E gene | RdRp IP2 | RdRp IP4 | Monitoring qRT‐PCR | Viral isolation | Viral neutralization titre | COVID‐19 positive contact | Animal symptoms |
| +C‐1 | Cat | Random/Madrid | Oropharyngeal swab | 32.17 | 30.14 | 33.03 | Positive (average Ct 32.78) | Yes* | Negative | No | History of diarrhoea secondary to chronic intestinal inflammation |
| Rectal swab | 34.84 | ND | 33.58 | ND | ND | ||||||
| Blood | ND | ND | ND | ND | X | ||||||
| +C‐2 | Cat | Selective/Castilla y León | Oropharyngeal swab | 31.99 | 30.78 | 30.18 | ND | Yes | 1/32 (serum = same day as positive PCR) | Yes | None |
| Rectal swab | ND | ND | ND | ND | X | ||||||
| Blood | ND | ND | ND | ND | X | ||||||
| +C‐3 | Cat | Selective/Castilla y León | Nasal swab | ND | 36.89 | 37.99 | ND | ND | Serum non‐available | Yes | None |
| Rectal swab | ND | ND | ND | ND | X | ||||||
| +C‐4 | Cat | Selective/Madrid | Oropharyngeal swab | 30.2 | ND | 30.8 | ND | ND |
1/64 (serum = same day as positive PCR) 1/32 (serum = 15 days after positive PCR) |
Yes | Leukaemia and feline immunodeficiency viruses positive Nasal discharge, anorexia, hypersomnia. Enlarged retropharyngeal lymph node |
Abbreviations: Ct, cycle threshold; ND, not detected.
*
Virus isolation was possible from the first swab collected.