FIG. 4.

Western blot analyses of the FimAs in five type-representative strains of P. gingivalis. Whole-cell lysates of P. gingivalis (2 × 10⁷ cells) were separated by SDS–12% polyacrylamide gel electrophoresis. After electrophoresis, gels were transferred to polyvinylidene difluoride membranes and FimA was detected with antibodies to rFimA (381, type I fimA [A], and HG564, type IV fimA [B]). Lanes: M, prestained protein marker; 1, P. gingivalis ATCC 33277; 2, P. gingivalis HW24D1; 3, P. gingivalis 6/26; 4, P. gingivalis HG564; 5, P. gingivalis HNA-99. Arrows indicate FimA.