Fig. 1.

Identification of metabolites and pathway analysis through different acquisition modes. (A) Histogram showing the distribution of the detected compounds among the T3-pos, T3-neg, amide-pos, and amide-neg analytical modes. (B) Histogram showing the distribution of the identified compounds among the T3-pos, T3-neg, amide-pos, and amide-neg analytical modes. (C) Venn diagram showing the distribution of the identified compounds among the T3-pos, T3-neg, amide-pos, and amide-neg analytical modes. (D) Metabolic pathways involving the identified metabolites in the control and NAFLD subjects were collected by the T3-pos, T3-neg, amide-pos, and amide-neg analytical modes. The larger the representative circle's size and the deeper the red color intensity, the greater the impact on the respective pathway. A total of 16 serum samples from NAFLD patients and healthy controls were acquired on LC-Q/TOF under different analytical modes, and datasets acquired under different analytical modes were subjected to data analysis and comparison. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)