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. 2021 Nov 26;12:777680. doi: 10.3389/fphar.2021.777680

FIGURE 2.

FIGURE 2

Effects of VP treatment on YAP/p-YAP expression levels following MCAO/R injury in mice. Mice were subjected to 1 h of ischemia and 24 h of reperfusion. (A) Representative western blots and quantitative analyses of the expression levels of p-YAP, YAP (cytoplasm), and YAP (nucleus) in vivo. (B) Representative microscopic images of YAP (green), based on immunofluorescence analyses. DAPI-stained nuclei are depicted in blue. The white arrow represents the number of YAP protein into the nucleus. Data are expressed as the mean ± SD, n = 6. ## p < 0.01 vs. Sham group, **p < 0.01 vs. MCAO/R group. Scale bar = 50 µm.