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. 2021 Aug 19;38(12):5453–5471. doi: 10.1093/molbev/msab245

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Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution 2021.

This work is written by US Government employees and is in the public domain in the US.

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Fig. 8. — Expression and evaluation of the antiretroviral activity of GagV1 and Pre-GagV1. (A) Immunoblotting analysis of HEK293T cells transfected with increasing amounts of plasmids expressing empty vector, GagV1-HA and Pre-GagV1-V5 was done using the indicated antibodies. (B, C) HIV-RenLuc or MLV-Luc produced in HEK293T cells following transfection with the indicated amounts of plasmids expressing GagV1-HA or Pre-GagV1-V5 was used to infect HELA (HIV-RenLuc) or NIH3T3 (MLV-Luc) cells. Normalized (B) Renilla or (C) firefly luciferase reporter values are shown relative to empty vector control. Error bars indicate standard deviation. Values represent the average of three independent experiments. (D) Immunoblotting analysis of whole cell lysate and pelleted supernatant from HEK293T cells transfected with GagV1-HA, MLV Gag-HA, or empty vector. Immunoblots are representative of at least two independent experiments.