Figure 4.

X-ray tomograms of cryopreserved PC3 human prostate cancer cells grown on Quantifoil TEM grids: (a) cell exposed to dark conditions (Figure S12, Video_T1); (b) cell exposed to blue light (465 nm) for 1 h (Figure S13, Video_T4); (c,d) cells exposed to 1× photoIC₅₀ (6.5 μM) of Pt2 for 2 h protected from light (Figures S14, S15, Video_T7, Video_T8). Distinct cellular features: (1) nucleus; (2) nuclear membrane; (3) mitochondria; (4) lipid droplets; (5) plasma membrane; (6) endosomes/lysosomes; (7) nucleolus; (8) dense organelles: Images were generated in IMOD software.⁶¹ No differences in cell morphology were observed between cells exposed to dark (a) or irradiated conditions (b). Multiple endosomes were observed in (c). Tiny black spots (high X-ray absorption) were observed in the nucleus of the cell mapped in (d) as indicated by blue boxes.