Figure 9.
Comparison of X-ray absorption near-edge structure (XANES) features for whole PC3 human prostate cancer cells treated with coumarin complex Pt2 in the dark or irradiated with blue light, acquired using the compressed-sensing method. Normalized spectra are shown of intracellular Pt in two independent 15 × 15 μm2 regions of cryo-fixed and dried PC3 cells treated with 5× photoIC50 (32.5 μM) of Pt2 in the dark (2 h protected from light, red) or irradiated (1 h exposure +1 h 465 nm, 17 J/cm2, blue) conditions. Data were acquired using 500 nm step size and 0.4 s dwell time. Spectra were analyzed in Athena XAS Data Analysis Software.71 Pre-edge normalization was performed in the energy range 11.47–11.54 keV, and the post-edge normalization was performed in the range 11.59–11.75 keV with a normalization order = 2. A shift in the Pt-edge peak to lower energies is observed upon irradiation with blue light, in addition to a reduction in peak height, suggesting that more Pt(II) is present upon irradiation.