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. 2021 Nov 26;15:771580. doi: 10.3389/fnins.2021.771580

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Copyright © 2021 Guida, Sanguigno, Mascolo, Calabrese, Serani, Molinaro, Lau, Annunziato and Formisano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SOD1 protein levels and cell viability in motor neuron like NSC34 cells transiently transfected with the empty vector, SOD1-WT and SOD1-G93A constructs. (A) Western Blot of SOD1 in NSC34 transiently transfected with the followed vectors: (1) empty vector (EV), (2) wild type human SOD1 (SOD1-WT) and (3) human SOD1 containing the G93A mutation (SOD1-G93A). Graphs show quantification of ratio of SOD1 and Actin. Bars represent mean ± SD (n = 3); *p ≤ 0.05 versus EV. (B) Effects of EV, SOD1-WT and SOD1-G93A vectors on cell survival, measured by MTT assay after 24, 48, and 72 h transfection. Bars represent mean ± SD (n = 3); *p ≤ 0.05 versus EV and SOD1-WT.