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. 2021 Nov 26;15:771580. doi: 10.3389/fnins.2021.771580

FIGURE 3.

FIGURE 3

Effect of REST knocking-down to counteract MeHg-induced cell death in SOD1-G93A motor neuron like NSC34 cells. (A,B,D,E,G,H) Effect of siRNA for REST (siREST) on REST protein levels, cell viability and LDH release in: (A,D,G) EV, and (B,E,H) SOD1-WT exposed to 72 h of MeHg (100 nM). Bars represent mean ± SD (n = 3). *p ≤ 0.05 versus vehicle (Veh), #p ≤ 0.05 versus siCTL + MeHg. (C,F,I) Effect of siRNA for REST (siREST) on REST protein levels, cell viability and LDH release in SOD1-G93A exposed to 24 h of MeHg (100 nM). Bars represent mean ± SD (n = 3). *p ≤ 0.05 versus vehicle (Veh), #p ≤ 0.05 versus siCTL + MeHg.