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. 2021 Nov 26;15:771580. doi: 10.3389/fnins.2021.771580

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Copyright © 2021 Guida, Sanguigno, Mascolo, Calabrese, Serani, Molinaro, Lau, Annunziato and Formisano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of: (1) REST knocking-down on RIPK1 and cleaved-caspase 8 protein levels and (2) Necrostatin-1 (Nec) on neurosurvival in SOD1-G93A motor neuron-like NSC34 cells exposed to MeHg (100 nM) at 24 h. (A,B) Western Blot of REST in MeHg-exposed SOD1-G93A cells transfected with siREST. Graph shows the quantification of the ratio of RIPK1 and cleaved-caspase 8 to tubulin. Bars represent mean ± SD (n = 3) per group. *p ≤ 0.05 versus Veh, #p ≤ 0.05 versus siCTL + MeHg 24 h. (C,D) MTT assay and LDH release in SOD1-G93A cells pre-treated for 2 h with Nec at 1, 5, 10, and 20 μM and with MeHg (100 nM) for 24 h. Bars represent mean ± SD (n = 3). *p ≤ 0.05 versus Veh, **p ≤ 0.05 versus MeHg, MeHg + Nec 1 μM, MeHg + Nec 5 μM.